#Fungal biocontrol agents series#

To develop liquid cultivation and formulation protocols that support the production of a stabilized biocontrol product that maintains superior levels of viability and efficacy over time in storage or in the field. Progress 04/24/03 to 05/13/07 Outputs Progress Report Objectives (from AD-416) To develop and execute commercial practice-oriented biocontrol agent selection strategies to discover agents for use against Fusarium head blight of wheat, potato storage diseases, or other emergent plant diseases that threaten U.S. Employ knowledge of stress response genes to design novel reporter-gene technologies, microbial cultivation protocols and down stream processing systems to foster and retain desired gene expression for surviving stress.

Stresses, especially those encountered during drying.

Utilize DNA micro array technology to discover genes involved in biocontrol agent cell response to environmental Use batch liquid cultivation techniques to develop biomass production media and protocols to maximize biomass quantity and quality efficacy survival of processing (dewatering and/or drying processes such as freeze-, air-, or spray-drying), formulation and storage and host compatibility. Industrial economics and market needs will be considered in crafting novel screens to select optimal biocontrol products and manufacturing processes. Strains or populations may be prescreened for a biological characteristic of known utility in supressing plant disease. Results suggest that the chamber system have the capability to meet the quick and simple biocontrol evaluation activities such as biological characteristic of various biocontrol agents, from synchronous production to multiplicate trials.Project Methods Depending on the pathosystem chosen for selecting biocontrol strains, whole microbial populations or individual candidate strains selected from key pathogen infection sites will be assayed against multiple pathogen strains using whole plant system bioassays that mimic natural infection conditions.

Moreover, conidial viability of those isolates was up to 95% entirely.

#Fungal biocontrol agents series#

Experimental data from the new series chamber system were obviously better than those of previous reports. anisopliae ARSEF 456, Beauveria bassiana ARSEF 734 and ARSEF 2860, Paecilomyces fumosoroseus ARSEF 6206 and ARSEF 3843 were assessed for the feasibility of chamber system. The conidial powder of those strains harvested from 5-kg rice cultures in a chamber weighed 147.1, 137.5, 101.2, 89.2, 55.0 and 40.0 g, and obtained 2.2

The synchronous conidia production of six fungal biocontrol candidates in three genera including Metarhizium flavoviride ARSEF 5628, M. A series solid-state fermentation (SSF) chamber system was designed to produce superior conidial powder of several fungal biocontrol agents.